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BIOTECH ELECTROPHORESIS Systems
PAPER ELECTROPHORESIS SYSTEMS
BioTech makes four different systems
for Paper Electrophoresis. They are usefull in diagonosis of certain diseases
like Multiple myeloma in clinical laboratories. These systems employ inexpensive
Whatman filter paper, agar/agarose gel or cellulose acetate membranes.
VERTICAL MODELS
Electrophoresis of 3 one inch/6 one inch width paper strips in a hanging inverted 'V' pattern or agar/agarose gel electrophoresis in micro-slides can be done. It is economical for routine laboratory investigations.
HORIZONTAL MODELS
Designed for the electrophoresis of 6 one inch width paper strips horizontally or 6 micro-slide gels. Experimental time can be reduced, because the strips length is reduced.
HORIZONTAL ELECTROPHORESIS SYSTEMS
BioTech offers 3 models of Horizontal Electrophoresis Systems. They are exclusively used for horizontal agar/agarose or starch gel electrophoresis. Serum proteins and other enzymes are separated. Immunological reagents are employed to achieve greater sensitivity, known as 'immunoelectrophoresis'.
STUDENT MODEL
It is designed to
carry out gel electrophoresis in two micro-slides. Buffer
required is about 100 ml hence highly economical. Minipack is sufficient
for running.
IMMUNO MODEL
A devoted system for all kinds of immuno electrophoresis in five micro -slides. The system can be placed in a refrigerator to maintain at low temperature in enzymic studies. A set of gel template and puncher are available with the system. Using the gel template, not only the gel can be cast in unifrom thickness but also sample wells can be made easy.
STARCH GEL SYSTEM
It is used for isozyme studies. Gel (20x19cm) casting, running can be carried out with ease & efficiency. Gel slicer helps to slice the gel of desired thickness.
SUBMARINE (HORIZONTAL) GEL SYSTEMS
Electrophoresis of nucleic acid fragments (DNA/RNA) are esentially carried out in agarose gels, Submerged under buffer, known as 'Submarine Gel Electrophoresis'. BioTech makes 5 different gel systems to cater all purposes. Every unit comes out of our laboratory spells out the following features.
* High buffer-gel ratio, to prevent gel melting & minimise pH difference during electrophoresis.
* Handy gel platforms, make gel easier; availability of UV-Transparent gel platforms help to view the brands without taking out.
* smooth well formation, using a variety of 'non-sticky' Teflon combs.
MINI MODEL
It is a compact unit for rapid analysis of plasmid DNA & restriction enzyme digests. Two different gel platforms are availabel. Well forming combs are made of Teflon.
ANALYTICAL MODEL
It is a versatile system. Three different sizes of gel platforms available. Restriction digests, fragment analysis and screening of plasmids can be done easily. Teflon combs as well as 30 sample can be loaded at a time.
PREPARATIVE MODEL
This is excllusively for preparative works-large scale isolation & purification of specific DNA fragment. This system is also used for the seperation of DNA fragments which requires longer run. The maximum gel size is 25x13 cm. Special acrylic combs in 6.0 mm or 3.0 mm thickness are available.
COMPACT MODEL
This is a student model with a two stage (50V or 100V) mini pack. This is an ideal system for teaching & demonstration of DNA electrophoresis. The floor of the system is made of UV transparent sheet. Gel can be cast on the floor itself and hence bands can be viewed without taking the gel out of the system. No separate gel platform is required.
MAXI MODEL
BioTech has designed a new extra large gel system to run several samples at high voltage. Built-in heat-exchanger and buffer circulation ports control 'joule' heat & ion depletion. So the system can be efficiently employed for conventional as well as field inversion nucleic acid separation techniques. Teflon combs upto 40 teeth are available for loading large number of samples.
ROD (TUBE/DISC) GEL
SYSTEMS
Polyacrylamide gel electrophoresis in glass tubes has got its own advantages. It is carried out even with a single samlpe very economically. Acrylamide gel polymerized in glass tubes are used, hence it is known as 'Tube Gel' electrophoresis. Sample is loaded on top of the gel and after the run the bands appear like, discs.
This technique (also known as DISC gel electrophoresis) is preferred for when there are only few samples. Both Rectangular and Circular models are available.
VERTICAL SLAB GEL SYSTEMS
One of the most widely used techniques
in Modern Biology is the 'Poly acrylamide gel electrophoresis (PAGE). Proteins,
Enzymes and other polypeptisides are studied. Electrophoresis in vertical
gel slabs has resulted in several advantages.
* Comparision of several samples can be done precisely in gel slabs than in rod gels.
* Use of thinner gels facilitates, faster staining & destaining of bands.
* Bands in the slab gels can be transferred onto membranes easily.
* Gel slabs can be dried for autoradiography & for preservation.
SEQUENCING
SYSTEM
Nowadays, separation of nucleotide fragments has become an essential step in DNA Sequencing. Electrophoretic separation is carried out in ultra-thin polyacrylamide gels. As the separation involves-handling of larger gels; employing high voltages and maintenance of specific thermal conditions, extreme care has been taken in designing our systems.
Two sequencing systems are available. Narrow Unit - 14x14 cm gel and Wider unit - 35x41 cm gel. They are made sturdy to hold heavy glass plates. Aluminium back plate is used to control temperature of the glass plates. Good quality gaskets, buffer drain ports ensure leak-free & safe operation of the systems.Gel is prepared outside using Teflon Combs (rectangular/shark tooth) & spacers are provided and as many as samples can be electrophoresed.
MULTI-PURPOSE ELECTROPHORESIS SYSTEMS
A
modular system for all kinds of Vertical & Horizontal electrophoresis
techniques. This system was designed in 1982 with the view of the growing
importance of Electrophoresis Techniques at college level. It is an all-in-one
model trained to perform every one of the following techniques with ease
and excellence.
* Horizontal agar/agarose gel electrophoresis in micro slide gels. * Immuno electrophoresis. * Electro focussing in horizontal poly acryllamide gel. * Starch gel electrophoresis in 13x13 cm gel. * Submarine gel electrophoresis in 13x13 cm gel. * Paper electrophoresis, 4 or 5 strips of 3x25 cm. * Rod gel electrophoresis. * Slab del electrophoresis in 16x14 cm gel.
ELECTRO-TRANSFER SYSTEMS
Electrophoresis systems described
as so far separate protiens or nucleic acids. Further studies
involve identification & characterization of bands,
for which, bands in the gels are extracted out and
concentrated. A kind of electrophoresis helps in this process known
as Electroelution. It is considered to be more efficient than
the other methods like dyalysis.
Very often, bands from the gel are transfered on to membranes, by electrophoresis known as "Electroblotting". They are then subjected to various analysis -very conveniently and efficiently.
BIOTECH makes compact systems - Electro Elutor and Bioblots for carrying out these techniques.
WET BLOTTERS
Transfer is carried out in Gel cassettes, totally submerged inthe buffer. Each cassette contains a gel & a membrane sandwiched inbetween scotch/cushion pads. As the membrane is facing towards anode, protiens/nucleic acids move towards membrane during run and get immobilized. Heat during run can be controlled by efficient heat-exchanger.
TRANSILLUMINATORS
UV TRANSILLUMINATORS - College Model
This unique
system is our own product, based on
"Dark-field" visualization. It is used to view Ethidium
Bromide stained nucleic acid bands. It has a viewing area of
25x15 cm. Suitable for most of the gels. UV-Transparent acrylic
sheet is placed in the viewing window, and
its background is darkened. UV lamps are mounted just below
the lid and kept in an angle so that
maximum light is focussed towards the window.
The unit does not employ the expensive Quartz filter & at the same time, visualization of bands are enhansed by the dark background. Hence, it is an economical system affordable by every laboratory.
TRANSILLUMINATORS - Research Models
BIOTECH introduces
2 models of new high sensitive Transilluminators. Long-life Quartz filter
is used. It is resistant to solarization by the UV light used. UV lamps are
mounted just below the Quartz filter to provide an intense & uniform
source of light. Cooling fan automatically runs when the UV is on, prevents
the unit thermal damages during prolonged usage.
TRANSILLUMINATORS - White
light
It illuminates with cool day light. Gels stained with visual dyes or autoradiography films are very clearly seen and photographed. It can be also used in graphic applications. It has 20x19 cm, window as viewing area, covered with opaque acrylic sheet to provide uniform intensity of light.
ELECTROPHORESIS POWER SUPPLIES
BIOTECH offers a wide range of Power supplies to perform various electrophoresis techniques. These are designed compact with sturdy components to provide troble free performances.
ANALOG MODEL POWER SUPPLIES
Besides the advanced
Digital Power Supplies, BIOTECH continues to make the earlier ALALOG MODEL
power packs on request. This is because of the time-proven customer satisfaction
and low costs. These power packs have been designed to deliver constant voltages.
Coarse & fine control knobs help to set it at the desired volts. Fuse
protects the unit from short- circuit / over loads conditions.
Analog Models power supplies are available in 3 models.
1. Analytical Model. 2. Vesatile Model. Hi-current MOdel.
ANALYTICAL MODEL
It
is designed for Electrophoresis requirement either
constant voltage (upto 300 DC) or current (upto 100 mA).
The automatic cross-over facility available controls the system within its
limit. SINGLE DIGITAL display and selector switch simplifies
the operation. Coarse & fine setting knobs help to
set the desired Volt & Current precisely. Utmost
safety and protection devices are also incorporated.
VERSATILE MODEL
To provide a wide
range of Power -upto 500V DC & 500mA. All the features are described
in the analytical model are available in this
and hence most of the Electrophoresis Techniques
can be performed using this single instrument.
HI-CURRENT MODEL
Especially, designed for experiments like, Electro-Blotting and Electro - Destaining. These techniques employ high current at low voltages. So this model with a current capacity upto 2.5A and voltage upto 250V serve the purpose well. All the Operational features & Safety measures are incorporated, as in the analytical model.
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